| Watershed | Sampling Method | Sample Date | Collector(s) | Determiner |
|---|---|---|---|---|
| Baskins Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Bradley Fork | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Bunches Creek | by hand | 1998-06-24 | I & M Aquatics Crew | Pennington & Associates |
| Bunches Creek | by hand | 1999-06-28 | I & M Aquatics Crew | American Aquatics |
| Bunches Creek | by hand | 2000-06-27 | I & M Aquatics Crew | American Aquatics |
| Bunches Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Cataloochee Creek | by hand | 2000-07-20 | I & M Aquatics Crew | American Aquatics |
| Cataloochee Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Cosby Creek | by hand | 1995-06-13 | GSalansky & HSoehn | Pennington & Associates |
| Deep Creek | by hand | 1997-08-28 | I & M Aquatics Crew | Pennington & Associates |
| East Prong Little River | by hand | 1993-06-15 | CRParker, SSummerall & DWilliams | Pennington & Associates |
| Hazel Creek | by hand | 1997-08-07 | I & M Aquatics Crew | Pennington & Associates |
| Hazel Creek | by hand | 2000-08-01 | I & M Aquatics Crew | American Aquatics |
| Hazel Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| LeConte Creek | by hand | 1995-06-01 | GSalansky & HSoehn | Pennington & Associates |
| LeConte Creek | by hand | 1996-11-27 | I & M Aquatics Crew | Pennington & Associates |
| LeConte Creek | by hand | 1997-09-15 | I & M Aquatics Crew | Pennington & Associates |
| Raven Fork | by hand | 1995-07-26 | GSalansky & HSoehn | Pennington & Associates |
| Twentymile Creek | by hand | 2000-07-10 | I & M Aquatics Crew | American Aquatics |
| Twentymile Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| West Prong Little Pigeon | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |