| Watershed | Sampling Method | Sample Date | Collector(s) | Determiner |
|---|---|---|---|---|
| Baskins Creek | by hand | 1998-06-16 | I & M Aquatics Crew | Pennington & Associates |
| Bunches Creek | by hand | 1998-06-15 | I & M Aquatics Crew | Pennington & Associates |
| Cataloochee Creek | at light | 2007-05-17 | ICStocks & EPT blitz 2 | CRParker |
| Cataloochee Creek | at light | 2007-05-17 | ICStocks & EPT blitz 2 | DAEtnier |
| Cataloochee Creek | by hand | 1984-08-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1986-07-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1989-07-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1990-01-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1990-07-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1990-10-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1991-07-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1997-07-01 | NC DEHNR | NC DEHNR |
| Cataloochee Creek | by hand | 1997-07-10 | I & M Aquatics Crew | Pennington & Associates |
| Cataloochee Creek | by hand | 1997-09-23 | I & M Aquatics Crew | Pennington & Associates |
| Cataloochee Creek | by hand | 1998-08-25 | I & M Aquatics Crew | Pennington & Associates |
| Cataloochee Creek | by hand | 1999-08-10 | I & M Aquatics Crew | American Aquatics |
| Cataloochee Creek | by hand | 1999-08-30 | I & M Aquatics Crew | American Aquatics |
| Cataloochee Creek | by hand | 2000-07-06 | I & M Aquatics Crew | American Aquatics |
| Cataloochee Creek | unspecified | 2002-07-02 | I & M Aquatics Crew | SChuluunbat |
| Cosby Creek | unspecified | 2002-06-17 | I & M Aquatics Crew | SChuluunbat |
| Deep Creek | by hand | 1958-09-13 | OSFlint Jr | OSFlint, Jr. |
| Forney Creek | by hand | 1994-07-20 | I & M Aquatics Crew | Pennington & Associates |
| Hazel Creek | by hand | 1998-08-18 | I & M Aquatics Crew | Pennington & Associates |
| Hazel Creek | unspecified | 2002-08-12 | I & M Aquatics Crew | SChuluunbat |
| Hazel Creek | unspecified | 2002-08-14 | I & M Aquatics Crew | SChuluunbat |
| LeConte Creek | by hand | 1998-06-04 | I & M Aquatics Crew | Pennington & Associates |
| Middle Prong Little Pigeon | unspecified | 2002-07-30 | I & M Aquatics Crew | SChuluunbat |
| Twentymile Creek | by hand | 1998-07-01 | I & M Aquatics Crew | Pennington & Associates |
| West Prong Little Pigeon | unspecified | 2002-06-18 | I & M Aquatics Crew | SChuluunbat |