| Watershed | Sampling Method | Sample Date | Collector(s) | Determiner |
|---|---|---|---|---|
| Abrams Creek | by hand | 1995-09-25 | GSalansky, CRParker & HSoehn | Pennington & Associates |
| Abrams Creek | by hand | 1995-09-26 | GSalansky, CRParker & HSoehn | Pennington & Associates |
| Abrams Creek | by hand | 1997-09-08 | I & M Aquatics Crew | Pennington & Associates |
| Abrams Creek | by hand | 1998-08-27 | I & M Aquatics Crew | Pennington & Associates |
| Abrams Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Abrams Creek | Malaise trap (Townes) | 2003-06-05 | BSutton & GSteck | CRParker |
| Abrams Creek | sweeping | 2001-06-25 | REDeWalt & BDHeinold | REDeWalt |
| Big Creek | by hand | 1999-08-09 | I & M Aquatics Crew | American Aquatics |
| Bradley Fork | by hand | 1997-06-26 | I & M Aquatics Crew | Pennington & Associates |
| Cataloochee Creek | by hand | 1994-07-12 | GSalansky & JLButcher | Pennington & Associates |
| Cataloochee Creek | by hand | 1994-07-22 | GSalansky & JLButcher | Pennington & Associates |
| Copeland Creek | by hand | 1994-06-14 | DAEtnier | DEtnier |
| Copeland Creek | by hand | 1996-07-23 | I & M Aquatics Crew | Pennington & Associates |
| Cosby Creek | by hand | 1993-06-17 | GSalansky & SSummerall | Pennington & Associates |
| Cosby Creek | by hand | 1994-06-01 | GSalansky & JLButcher | Pennington & Associates |
| Cosby Creek | by hand | 1997-06-18 | I & M Aquatics Crew | Pennington & Associates |
| Cosby Creek | by hand | 1998-06-22 | I & M Aquatics Crew | Pennington & Associates |
| Dudley Creek | by hand | 1996-09-04 | I & M Aquatics Crew | Pennington & Associates |
| East Prong Little River | by hand | 1994-09-14 | GSalansky & JLButcher | Pennington & Associates |
| East Prong Little River | by hand | 1995-06-15 | GSalansky & HSoehn | Pennington & Associates |
| East Prong Little River | by hand | 1995-09-19 | GSalansky & HSoehn | Pennington & Associates |
| East Prong Little River | by hand | 2000-08-09 | I & M Aquatics Crew | American Aquatics |
| East Prong Little River | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Forney Creek | by hand | 1994-06-28 | I & M Aquatics Crew | Pennington & Associates |
| Hazel Creek | by hand | 1997-08-04 | I & M Aquatics Crew | Pennington & Associates |
| Hazel Creek | by hand | 1999-07-19 | I & M Aquatics Crew | American Aquatics |
| Hazel Creek | by hand | 2000-07-31 | I & M Aquatics Crew | American Aquatics |
| Hesse Creek | by hand | 1997-07-17 | I & M Aquatics Crew | Pennington & Associates |
| LeConte Creek | by hand | 1995-06-01 | GSalansky & HSoehn | Pennington & Associates |
| LeConte Creek | by hand | 1996-07-18 | I & M Aquatics Crew | Pennington & Associates |
| LeConte Creek | by hand | 1996-11-27 | I & M Aquatics Crew | Pennington & Associates |
| Panther Creek | by hand | 1994-09-21 | GSalansky & JLButcher | Pennington & Associates |
| Raven Fork | by hand | 1995-07-27 | GSalansky & HSoehn | Pennington & Associates |
| Twentymile Creek | by hand | 1999-06-23 | I & M Aquatics Crew | American Aquatics |
| Twentymile Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| West Prong Little Pigeon | by hand | 1995-05-25 | GSalansky | Pennington & Associates |
| West Prong Little Pigeon | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |