| Watershed | Sampling Method | Sample Date | Collector(s) | Determiner |
|---|---|---|---|---|
| Abrams Creek | by hand | 1993-12-15 | SJFraley & DAEtnier | SJFraley |
| Bradley Fork | by hand | 1967-10-02 | TYamamoto & AOdum | OSFlint, Jr. |
| Deep Creek | by hand | 1939-08-16 | THowell | OSFlint, Jr. |
| Deep Creek | by hand | 1958-09-13 | OSFlint Jr & GBWiggins | OSFlint, Jr. |
| Deep Creek | by hand | 1994-06-06 | NC DEHNR | NC DEHNR |
| Deep Creek | by hand | 1997-07-22 | I & M Aquatics Crew | Pennington & Associates |
| Deep Creek | unspecified | 1970-05-21 | OSFlint Jr | OSFlint, Jr. |
| East Prong Little River | by hand | 1994-09-14 | GSalansky & JLButcher | Pennington & Associates |
| East Prong Little River | by hand | 1996-09-17 | I & M Aquatics Crew | Pennington & Associates |
| East Prong Little River | by hand | 1999-09-01 | I & M Aquatics Crew | American Aquatics |
| East Prong Little River | by hand | 2000-08-17 | I & M Aquatics Crew | American Aquatics |
| East Prong Little River | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Forney Creek | by hand | 1994-06-06 | NC DEHNR | NC DEHNR |
| Hazel Creek | by hand | 1999-07-21 | I & M Aquatics Crew | American Aquatics |
| Hazel Creek | by hand | 2000-07-31 | I & M Aquatics Crew | American Aquatics |
| Hazel Creek | by hand | 2000-08-01 | I & M Aquatics Crew | American Aquatics |
| Hazel Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Middle Prong Little River | by hand | 2005-05-02 | CRParker & JRobinson | CRParker |
| Oconaluftee River, Lower | by hand | 1999-09-26 | MJWetzel & MAPMorgan | CRParker |
| Twentymile Creek | by hand | 1996-08-21 | I & M Aquatics Crew | Pennington & Associates |
| Twentymile Creek | by hand | 2000-07-10 | I & M Aquatics Crew | American Aquatics |
| Twentymile Creek | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |